Surface-Plasmon-Mediated Single-Molecule Fluorescence Through a Thin Metallic Film

F. D. STEFANI; K. VASILEV; N. BOCCHIO; N. STOYANOVA; M. KREITER

PHYSICAL REVIEW LETTERS

AMER PHYSICAL SOC

Año: 2005 vol. 94 p. 230051 - 230054

0031-9007

We demonstrate that fluorescence of single molecules in the nanometric vicinity of a thin gold film can be effectively excited and detected through the film with an epi-illumination scanning confocal micro- scope. A full theoretical treatment of the fluorescence signal indicates that both excitation and emission are surface-plasmon mediated. Remarkably, the number of photons detectable from chromophores perpendicular to the interface is enhanced by the presence of the metal.