INVESTIGADORES
STEFANI Fernando Daniel
artículos
Título:
Impact of micropatterned surfaces on neuronal polarity
Autor/es:
ANGELA KATRIN VOGT; FERNANDO DANIEL STEFANI; ANDREAS BEST; GABRIELE NELLES; AKIO YASUDA; WOLFGANG KNOLL; ANDREAS OFFENHÄUSSER
Revista:
JOURNAL OF NEUROSCIENCE METHODS
Editorial:
ELSEVIER SCIENCE BV
Referencias:
Año: 2004 vol. 134 p. 191 - 198
ISSN:
0165-0270
Resumen:
Experimental control over cellular polarity in a neuronal network is a
promising tool to study synapse formation and network behavior. We
aimed to exploit a mechanism described by Stenger et al. [J. Neurosci.
Methods 82 (1998) 167] to manipulate the direction of axonal versus
dendritic outgrowth on a micropattern. The group had used laser
ablation to create patterns of aminated silanes for cell attachment on
a background of repellent fluorinated silanes. The pattern offered
continuous adhesive pathways for axonal and interrupted pathways for
dendritic outgrowth. By microcontact printing, we created similar
patterns containing continuous and interrupted pathways consisting of
extracellular matrix proteins on a background of polystyrene. Neuronal
polarity was determined on the functional level through double patch
clamp measurements, detecting synapses and their orientation. Although
our pattern reproduced the properties that were assumed to be critical
for the described effect, namely contrasting pathways of different
adhesiveness, we failed to reproduce the above results. It is indicated
that other qualities of alternative pathways than mere differences in
adhesiveness are required to orient neuronal polarity in vitro. We
suggest that the effect observed by Stenger et al. has to be attributed
to less universal characteristics of the micropattern, e.g. to the
specific chemical groups that were utilized.