Peptide Affinity Chromatography Applied to Therapeutic Antibodies Purification

BARREDO-VACCHELLI, G.R.; GIUDICESSI, S.L.; MARTÍNEZ-CERON, M.C.; CASCONE, O,; CAMPERI, S.A.

International Journal of Peptide Research and Therapeutics

Springer

Año: 2021

The interest in therapeutic monoclonal antibodies (mAbs) has signifcantly grown in the pharmaceutical industry, exceeding 100 FDA mAbs approved. Although the upstream processing of their industrial production has been signifcantly improved in the last years, the downstream processing still depends on immobilized protein A afnity chromatography. The high cost, low capacity and short half-life of immobilized protein A chromatography matrices, encouraged the design of alternative short-peptide ligands for mAb purifcation. Most of these peptides have been obtained by screening combinatorial peptide libraries. These low-cost ligands can be easily produced by solid-phase peptide synthesis and can be immobilized on chromatographic supports, thus obtaining matrices with high capacity and selectivity. Furthermore, matrices with immobilized peptide ligands have longer half-life than those with protein A due to the higher stability of the peptides. In this review the design and synthesis of peptide ligands, their immobilization on chromatographic supports and the evaluation of the afnity supports for their application in mAb purifcation is described.