Abstract: LACTIC ACID BACTERIA AND BIFIDOBACTERIA FROM A BULLFROG HATCHERY IN CORDOBA: EVALUATION OF BENEFICIAL CHARACTERISTICS

MONTEL MENDOZA, G.; ALE, C. E.; PASTERIS, S.E.; OTERO, M.C.; BUHLER, M.I.; NADER-MACÍAS, M. E.

BIOCELL

INST HISTOL EMBRIOL-CONICET

Lugar: Mendoza; Año: 2010 vol. 32 p. 161 - 161

0327-9545

Rana catesbeiana is the most appropriate species for raniculture, where diseases such as the red leg syndrome (RLS) contribute to animal mass mortality with a high impact on production costs. The use of probiotics is becoming increasingly popular in aquaculture,Lactic Acid Bacteria (LAB) being the most suitable microorganisms for this purpose. In this work we evaluated the beneficial properties (production of antagonistic compounds and surface properties) of 52 LAB and 5 Bifidobacterium sp. isolated from a hatchery in Córdoba and their differences with the LAB isolated from a Northwestern hatchery. Results showed that all the strains inhibited at least one of the pathogens assayed by organic acid (agar well diffusion method). Sixteen strains were found to produce H2O2 in a specific medium (MRS+TMB+peroxidase). Only one strain produced a bacteriocin active against S. epidermidis and L. monocytogenes. Surface properties assays showed that most of the strains were hydrophilic. However, 8 strains exhibited a high hydrophobicity percentage, while 5 were autoagreggative. Bifidobacteria presented hydrophilic properties and only one showed antagonist activity. LAB isolated from a Northwestern hatchery have inhibitory and surface properties similar to those from Córdoba. However, the percentage of H2O2-producing strains was higher. The results obtained allow us to select some potential probiotic strains to be used in different aquaculture areas according to the biodiversity found.