Characterization of Lasiodiplodia species associated with grapevines in Mexico

RANGEL-MONTOYA EDELWEISS; PAOLINELLI, MARCOS; ROLSHAUSEN PHILIPPE; VALENZUELA-SOLANO, CESAR; HERNÁNDEZ-MARTÍNEZ RUFINA

PHYTOPATHOLOGIA MEDITERRANEA

MEDITERRANEAN PHYTOPATHOLOGICAL UNION

Lugar: Firenze- Italia; Año: 2021

0031-9465

Botryosphaeria dieback is one of the most prevalent grapevine trunk diseases (GTDs) caused by fungi from the Botryosphaeriaceae family. Fungi invade the vascular system mainly through pruning wounds and cause cankers and necrotic lesions, which lead to grapevine decline and death. Lasiodiplodia theobromae has been reported as a highly virulent pathogen on grapevine and was previously reported in Mexican vineyards. The taxonomy of Lasiodiplodia genus was recently revised, adding new species, and some were reduced to synonymy. This work aimed to characterize fungi from the Lasiodiplodia genus producing grapevine dieback symptoms in Sonora and Baja California, Mexico. Using the phylogenetic markers tef1-α and ITS regions, Lasiodiplodia brasiliensis, L. crassispora, L. exigua, and L. gilanensis were identified. Lasidiplodia exigua was the most prevalent species. L. brasiliensis and L. gilanensis showed high virulence in grapevine plants cv. Cabernet Sauvignon while L. exigua and L. gilanensis were less virulent, and L. crassispora did not produce lesions at two months post-inoculation. The optimum temperature of the Lasiodiplodia spp. was 28 °C, but all species were able to grow up to 37 °C, and the isolates of L. exigua grew slowly at 40°C. To our best knowledge, this is the first report of the four of Lasiodiplodia species in vineyards of Mexico.