Genotyping of Trypanosoma cruzi sub-lineage in human samples from a north-east Argentina area by hybridization with DNA probes and specific PCR

DIEZ CRISTINA; LORENZ VIRGINIA; ORTIZ SILVIA; GONZALEZ VERÓNICA; RACCA ANDREA; BONTEMPI IVAN; MANATTINI SILVIA; SOLARI ALDO; MARCIPAR IVAN

AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE

AMER SOC TROP MED & HYGIENE

Año: 2010 vol. 82 p. 63 - 73

0002-9637

We have evaluated blood samples of chronic and congenital Trypanosoma cruzi -infected patients from the city of Reconquista located in the northeast of Argentina where no information was previously obtained about the genotype of infecting parasites. Fourteen samples of congenital and 19 chronical patients were analyzed by hybridization with DNA probes of minicircle hypervariable regions (mHVR). In congenital patients, 50% had single infections with TcIId, 7% single infections with TcIIe, 29% mixed infections with TcIId/e, and 7% had mixed infections with TcIId/b and 7%TcIId/b, respectively. In Chronical patients, 52% had single infections with TcIId, 11% single  infections with TcIIe, 26% had mixed infections with TcIId/e, and 11% had non-identified genotypes. With these samples, we evaluated the minicircle lineage-specific polymerase chain reaction assay (MLS-PCR), which involves a nested PCR to HVR minicircle sequencesand we found a correlation with hybridization probes of 96.4% for TcIId and 54.8% for TcIIe.