Genome Sequence and Characterization of Lactobacillus casei Phage, vB_LcaM_Lbab1 Isolated from Raw Milk

GRADASCHI, VICTORIA; PAYASLIAN, FLORENCIA; DIETERLE, MARIA EUGENIA; RONDÓN SALAZAR, LILIANA; URDÁNIZ, ESTEFANÍA; DI PAOLA, MATIAS; PEÑA CÁRCAMO, JOSÉ; ZON, FABIO; ALLIEVI, MARIANA; SOSA, EZEQUIEL; FERNANDEZ DO PORTO, DARÍO; DUNNE, MATTHEW; GOELLER, PAULINE; KLUMPP, JOCHEN; RAYA, RAÚL RICARDO; REYES, ALEJANDRO; PIURI, MARIANA

PHAGE

Mary Ann Liebert

Año: 2021

2641-6530

Introduction: There are few Lactobacillus casei phages characterized so far. Several L. casei strains are part of probiotic formulations so phage attack can generate important losses in the dairy industry. Materials and Methods: A new phage of L. casei was isolated from raw milk obtained from a milking yard from Buenos Aires province. Phage genome was sequenced, annotated, and analyzed. Morphology was determined by electron microscopy and its host range was established.Results: Lactobacillus phage vB_LcaM_Lbab1 is a Myovirus and belongs to the Herelleviridae family and its host range includes L. casei/paracasei strains and L. kefiri. We further analyzed the baseplate proteins in silico and found putative Carbohydrate Binding Modules (CBM), responsible for host recognition in other Lactobacillus phages.Conclusions: A new Lactobacillus phage was isolated and characterized. The focus was made on its host recognition mechanism, pointing towards the development of future strategies to avoid deleterious infections in the dairy industry.