An all-inclusive and straightway laboratory activity to solve the 3D crystal structure of a protein

KLINKE, SEBASTIÁN; RINALDI, JIMENA; GOLDBAUM, FERNANDO A.; SUAREZ, SEBASTIÁN; OTERO, LISANDRO H. * (* CORRESPONDING AUTHOR)

BIOCHEMISTRY AND MOLECULAR BIOLOGY EDUCATION

JOHN WILEY & SONS INC

Lugar: New York; Año: 2019 vol. 47 p. 700 - 707

1470-8175

X-raycrystallography provides structural information of molecules at theatomic level, being a central technique at the forefront of scienceand technology. However, crystallography teaching is not usuallyimplemented in biochemistry lab classes due to its complex executionby non-expert users. Here, we report the basic step-by-step workflowperformed by crystallographers in order to solve the 3D structure ofa protein. All these activities were executed in a course forLatin-American graduate students with no previous knowledge on X-raycrystallography entitled ?Crystallography in Structural Biology:why do we need a protein crystal, and how do we get it??.We would like to share our experience with the educational researchcommunity, with the main purpose being to enrich teaching inbiochemistry and structural molecular biology by performing a seriesof interesting laboratory and computer experiments.p { margin-bottom: 0.25cm; line-height: 115%; }p { margin-bottom: 0.25cm; line-height: 115%; }