INGEBI 02650
INSTITUTO DE INVESTIGACIONES EN INGENIERIA GENETICA Y BIOLOGIA MOLECULAR "DR. HECTOR N TORRES"
Unidad Ejecutora - UE
artículos
Título:
In vitro differentiation of Trypanosoma cruzi epimastigotes into metacyclic trypomastigotes using a biphasic medium
Autor/es:
MUÑOZ-CALDERÓN, ARTURO; GÓMEZ, KARINA ANDREA; RODRÍGUEZ DURÁN, JESSICA; POTENZA, MARIANA
Revista:
STAR Protocols
Editorial:
Cell Press
Referencias:
Año: 2021 vol. 2
ISSN:
2666-1667
Resumen:
The pathogen Trypanosoma cruzi differentiates from epimastigotes (E) intoinfective metacyclic trypomastigotes (MTs) to invade the mammalian cell. Thisprocess, called metacyclogenesis, is mimicked in vitro by nutrient starvation orincubation with minimal media. Here, we describe an alternative protocol formetacyclogenesis by incubating E forms in a biphasic medium supplementedwith human blood. Although time consuming, this procedure yields fully differentiatedMTs without the presence of intermediate forms, even for cultures thathave been maintained as E for years.
