Synthesis and Characterization of Heparin-Doped Polypyrrole Coatings Using an Electrochemical Quartz Crystal Microbalance (EQCM)

DANIEL OMAR FLAMINI; GONZÁLEZ, MARÍA BELÉN; SILVANA BEATRIZ SAIDMAN

PORTUGALIAE ELECTROCHIMICA ACTA

Sociedade Portuguesa de Electroquimica

Año: 2022

0872-1904

Polypyrrole (PPy) films were electrochemically synthesized on Au/TiO2 coated quartz crystal electrode in solutions with different concentrations of heparin (Hep). The morphology of the PPy films was determined by tapping mode atomic force microscopy (AFM). The influence of the dopant concentration on the coating surface roughness was studied. Electrochemical quartz crystal microbalance (EQCM) results suggest that the Hep retained in the PPy films is binding to thrombin. The amount of adsorbed thrombin is increased with higher coating surface roughness. PPy films doped with Hep are electroactive and show cation exchange properties under oxidation or reduction conditions in Ringer solution. Pre-oxidized PPy film adsorbs a greater amount of thrombin than the freshly one and even more in relation to the pre-reduced film.