INVESTIGADORES
VARONE Cecilia Laura
artículos
Título:
Activated Translation Signaling in Placenta from Pregnant Women with Gestational Diabetes Mellitus: Possible Role of Leptin.
Autor/es:
ANTONIO PÉREZ PÉREZ; JULIETA L. MAYMÓ; YÉSICA GAMBINO; PILAR GUADIX; JOSÉ DUEÑAS; CECILIA L. VARONE; VÍCTOR SÁNCHEZ-MARGALET
Revista:
HORMONE AND METABOLIC RESEARCH
Editorial:
GEORG THIEME VERLAG KG
Referencias:
Año: 2013 vol. 45 p. 436 - 442
ISSN:
0018-5043
Resumen:
Placentas from gestational diabetes (GDM) suff er
from structural and functional changes including
overgrowth. That is why we aimed to study
[ 3 H]-leucine incorporation into protein in addition
to translation signaling in placenta from
GDM. Thus, we investigated the expression of
leptin and leptin receptor (LEPR), as well as the
activation state of signaling proteins regulating
protein synthesis, such as mTOR, S6 Kinase,
EIF4E-BP1, EIF4E, and eEF2 by measuring protein
phosphorylation by immunoblot. [ 3 H]-Leucine
incorporation into protein also was determined
in trophoblastic placenta explants from GDM
and control pregnancy. We found that leptin and
LEPR expression are increased in placentas from
GDM and the translation machinery activity as
well as [ 3 H]-leucine incorporation into protein
were higher in placentas from GDM compared
with placentas from control pregnancy. In conclusion,
protein synthesis rate is increased in
placenta from GDM patients, and this may be
due, at least in part, by the activation of translation
signaling. The increased expression of leptin
and LEPR may contribute to these eff ects. These
results may provide a possible mechanism for
the previously observed increase in placenta
growth in GDM.ff er
from structural and functional changes including
overgrowth. That is why we aimed to study
[ 3 H]-leucine incorporation into protein in addition
to translation signaling in placenta from
GDM. Thus, we investigated the expression of
leptin and leptin receptor (LEPR), as well as the
activation state of signaling proteins regulating
protein synthesis, such as mTOR, S6 Kinase,
EIF4E-BP1, EIF4E, and eEF2 by measuring protein
phosphorylation by immunoblot. [ 3 H]-Leucine
incorporation into protein also was determined
in trophoblastic placenta explants from GDM
and control pregnancy. We found that leptin and
LEPR expression are increased in placentas from
GDM and the translation machinery activity as
well as [ 3 H]-leucine incorporation into protein
were higher in placentas from GDM compared
with placentas from control pregnancy. In conclusion,
protein synthesis rate is increased in
placenta from GDM patients, and this may be
due, at least in part, by the activation of translation
signaling. The increased expression of leptin
and LEPR may contribute to these eff ects. These
results may provide a possible mechanism for
the previously observed increase in placenta
growth in GDM.3 H]-leucine incorporation into protein in addition
to translation signaling in placenta from
GDM. Thus, we investigated the expression of
leptin and leptin receptor (LEPR), as well as the
activation state of signaling proteins regulating
protein synthesis, such as mTOR, S6 Kinase,
EIF4E-BP1, EIF4E, and eEF2 by measuring protein
phosphorylation by immunoblot. [ 3 H]-Leucine
incorporation into protein also was determined
in trophoblastic placenta explants from GDM
and control pregnancy. We found that leptin and
LEPR expression are increased in placentas from
GDM and the translation machinery activity as
well as [ 3 H]-leucine incorporation into protein
were higher in placentas from GDM compared
with placentas from control pregnancy. In conclusion,
protein synthesis rate is increased in
placenta from GDM patients, and this may be
due, at least in part, by the activation of translation
signaling. The increased expression of leptin
and LEPR may contribute to these eff ects. These
results may provide a possible mechanism for
the previously observed increase in placenta
growth in GDM.3 H]-Leucine
incorporation into protein also was determined
in trophoblastic placenta explants from GDM
and control pregnancy. We found that leptin and
LEPR expression are increased in placentas from
GDM and the translation machinery activity as
well as [ 3 H]-leucine incorporation into protein
were higher in placentas from GDM compared
with placentas from control pregnancy. In conclusion,
protein synthesis rate is increased in
placenta from GDM patients, and this may be
due, at least in part, by the activation of translation
signaling. The increased expression of leptin
and LEPR may contribute to these eff ects. These
results may provide a possible mechanism for
the previously observed increase in placenta
growth in GDM.3 H]-leucine incorporation into protein
were higher in placentas from GDM compared
with placentas from control pregnancy. In conclusion,
protein synthesis rate is increased in
placenta from GDM patients, and this may be
due, at least in part, by the activation of translation
signaling. The increased expression of leptin
and LEPR may contribute to these eff ects. These
results may provide a possible mechanism for
the previously observed increase in placenta
growth in GDM.ff ects. These
results may provide a possible mechanism for
the previously observed increase in placenta
growth in GDM.