Ghrelin antagonist D‐Lys3‐GHRP‐6 counteract ghrelin effects in bovine cumulus‐oocytes complexes matured in vitro

CARRANZA?MARTÍN, ANA C.; NIKOLOFF, NOELIA; ANCHORDOQUY, J. PATRICIO; ANCHORDOQUY, J. MATEO; RELLING, ALEJANDRO E.; FURNUS, CECILIA C.

REPRODUCTION IN DOMESTIC ANIMALS (1990)

WILEY-BLACKWELL PUBLISHING, INC

Lugar: Londres; Año: 2021

0936-6768

Ghrelin is a gut hormone related to energy balance and reproductive functions. Theaim of this study was to evaluate the effect of ghrelin antagonist D-Lys3-GHRP-6(GA) as a potential agent that prevents ghrelin effects during bovine oocyte maturationon progesterone production, cumulus cell (CC) viability, CC DNA damage andembryo development and hatching rates. Ghrelin´s potential to induce oxidativestress in cumulus-oocytecomplexes (COC) was also evaluated. COCs were culturedfor 24 hr in medium without supplementation (C) or supplemented with 60 pM ghrelin(Ghrelin60), Ghrelin60 + 20 pM GA (GA20), Ghrelin60 + 60 pM GA (GA60) orGhrelin60 + 100 pM GA (GA100) for experiment I. For experiment II, C and Ghrelin60treatments were used. Differences between C and Ghrelin60 and the linear or quadraticassociation between GAs on Ghrelin60 were evaluated. Results demonstratedthat Ghrelin60 increased progesterone concentration, reduced CC viability, inducedCC DNA damage and decreased blastocyst and hatching rate compared with C(p < .05). GA20, GA60 and GA100 had a linear effect on CC genetic damage index(p ≤ .05) and a quadratic effect on CC viability (p < .01). GA20 counteracted the lowhatching rate produced by Ghrelin60. However, GAs did not counteract progesteroneconcentration and blastocyst rate (p ≥ .21). GRH60 did not differ from C in theoxidative status (p ≥ .19). Our study highlights that GA could prevent the negativeeffects of ghrelin during bovine IVM.