Plant L10 ribosomal proteins have different roles during development and translation under UV-B stress

MARIA LORENA FALCONE FERREYRA; ALEJANDRO PEZZA; JORDANE BIARC; ALMA L. BURLINGAME; PAULA CASATI

PLANT PHYSIOLOGY.

AMER SOC PLANT BIOLOGISTS

Año: 2010 vol. 153 p. 1878 - 1894

0032-0889

RPL10 proteins are ubiquitous in the plant kingdom, Arabidopsis has three RPL10 genes encoding RPL10A-C proteins, while two genes are present in the maize genome (rpl10-1 and -2). Maize and Arabidopsis thaliana RPL10s are tissue specific and developmentally regulated, showing high levels of expression in tissues with active cell division. Co-immunoprecipitation experiments indicate that RPL10s in Arabidopsis associate with translation proteins, demonstrating it is a component of the 80S ribosome. Previously, UV-B exposure was shown to increase the expression of a number of maize ribosomal protein genes, including rpl10. In this work, we demonstrate that maize rpl10 genes are induced by UV-B while At RPL10s are differentially regulated by this radiation: RPL10A is not UV-B regulated, RPL10B is down-regulated, while RPL10C is up-regulated by UV-B in all organs studied. Characterization of Arabidopsis T-DNA insertional mutants indicates that RPL10 genes are not functionally equivalent. rpl10A and rpl10B mutant plants show different phenotypes: knockout rpl10A mutants are lethal, rpl10A heterozygous plants are deficient in translation under UV-B conditions, and knockdown homozygous rpl10B mutants show abnormal growth. Based on the results described here, RPL10 genes are not redundant and participate in development and translation under UV-B stress.