Release of ATP by TRPV4 activation is dependent upon the expression of AQP2 in renal cells

PIZZONI, ALEJANDRO; BAZZI, ZAHER; DI GIUSTO, GISELA; ALVAREZ, CORA L.; RIVAROLA, VALERIA; CAPURRO, CLAUDIA; SCHWARZBAUM, PABLO J.; FORD, PAULA

JOURNAL OF CELLULAR PHYSIOLOGY

WILEY-LISS, DIV JOHN WILEY & SONS INC

Lugar: Hoboken; Año: 2020

0021-9541

Increasing evidence indicates that aquaporins (AQPs) exert an influence in cellsignaling by the interplay with the transient receptor potential vanilloid 4 (TRPV4)channel. We previously found that TRPV4 physically and functionally interacts withAQP2 in cortical collecting ducts (CCD) cells, favoring cell volume regulation and cellmigration. Because TRPV4 was implicated in ATP release in several tissues, weinvestigated the possibility that TRPV4/AQP2 interaction influences ATP release inCCD cells. Using two CCD cell lines expressing or not AQP2, we measured extra-cellular ATP (ATPe) under TRPV4 activation and intracellular Ca2+under ATPaddition. We found that AQP2 is critical for the release of ATP induced by TRPV4activation. This ATP release occurs by an exocytic and a conductive route. ATPe, inturn, stimulates purinergic receptors leading to ATPe‐induced ATP release by aCa2+‐dependent mechanism. We propose that AQP2 by modulating Ca2+and ATPdifferently could explain AQP2‐ increased cell migration