INGEBI   02650
INSTITUTO DE INVESTIGACIONES EN INGENIERIA GENETICA Y BIOLOGIA MOLECULAR "DR. HECTOR N TORRES"
Unidad Ejecutora - UE
artículos
Título:
Angiotensin-(1-7) upregulates cardiac nitric oxide synthase in spontaneously hypertensive rats.
Autor/es:
MARIA DE LOS ANGELES COSTA; MARÍA A. LOPEZ VERRILLI; KARINA GOMEZ; PABLO NAKAGAWA; CLARA PEÑA; CRISTINA ARRANZ,; MARIELA GIRONACCI
Revista:
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
Editorial:
AMER PHYSIOLOGICAL SOC
Referencias:
Lugar: Bethesda; Año: 2010 vol. 299 p. 1205 - 1211
ISSN:
0363-6135
Resumen:
It has been shown that angiotensin (ANG)-(1–7) activates nitric oxide synthase (NOS) in isolated ventricular myocytes from normotensive rats. Since many ANG-(1–7) actions are enhanced in situations of increased ANG II activity, as in hypertension, in this study we investigated the in vivo effect of ANG-(1–7) on NOS activity and expression of endothelial (eNOS), neuronal (nNOS), and inducible NOS (iNOS) in ventricles from spontaneously hypertensive rats (SHR). Rats were subjected to a 60-min ANG-(1–7) infusion (0.35 nmol/min); controls received saline. NOS activity was measured using the NADPH diaphorase histochemical method and by the conversion of L-[14C]arginine to citrulline, and NOS phosphorylation and expression were determined using Western blotting. In SHR, ANG-(1–7) infusion diminished mean arterial pressure from 180 +/- 9 to 146 +/- 9 mmHg (P +/- 0.05), and this effect was prevented by nitro-L-arginine methyl ester (LNAME), a NOS inhibitor. In addition, NOS activity and eNOS phosphorylation were increased by ANG-(1–7) infusion. Ventricular eNOS and nNOS expression were increased 67.4 +/- 6.4 and 51 +/- 10%, respectively, by ANG-(1–7), whereas iNOS was not changed. In another set of experiments, we evaluated the mechanism by which ANG-(1–7) modifies NOS activity. Isolated ventricle slices preincubated with ANG-(1–7) showed an increase in NOS activity and eNOS phosphorylation, which was blocked by an AT2 and a bradykinin B2 receptor antagonist, but not by the Mas receptor antagonist. Our results show that in rats in a hypertensive state, ANG-(1–7) infusion upregulates cardiac NOS expression and activity through an AT2- and bradykinindependent mechanism. In this way ANG-(1–7) may elicit its cardioprotective action and contribute to some of the counterregulatory AT2 receptor effects that oppose the AT1 receptor-mediated effects.