Na+, K+-ATPase was found to be the membrane component responsible for the hodrofobic behavior of the brain membrane tubulin

ALONSO A. C.; NUÑEZ-FERNANDEZ M.; BELTRAMO D. M.; CASALE C. H.; BARRA H. S.

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Año: 1998 vol. 253 p. 824 - 827

0006-291X

We have previously described that the tubulin isolated from brain membranes as a hydrophobic compound by partitioning into Triton X-114. The hydrophobic behavior of this tubulin is due to its interaction with membrane protein(s) and the interaction occurs principally with the acetylated tubulin isotype. In the present work we identified the membrane protein that interacts with tubulin as the Na1,K1-ATPase a subunit by amino acid sequencing. Using purified brain Na1,K1-ATPase we were able to isolate part of the total hydrophilic tubulin as a hydrophobic compound which contains a high proportion of the acetylated tubulin isotype.