Isolation of culturable mycota from Argentinean soils exposed and no-exposed to pesticides and determination of glyphosate tolerance of fungal species in supplied media with the herbicide

ALUFFI M.E.; CARRANZA C.S.; BENITO N.; MAGNOLI K.; MAGNOLI C.E,; BARBERIS C.L.

REVISTA ARGENTINA DE MICROBIOLOGíA

ASOCIACION ARGENTINA MICROBIOLOGIA

Lugar: Buenos Aires; Año: 2020 vol. 52 p. 221 - 230

0325-7541

The current agricultural system led to the development of glyphosate (GP) resistant weeds, causing an increase in GP doses and applications. Native mycota of pesticides contaminated sites are the major source of pesticide-degrading microorganisms. The aims of the present study were to isolate the GP tolerant culturable mycota in two soils with different pesticides exposition from Córdoba, Argentina, and to evaluate in native fungal isolates the growth parameters in presence of GP and the effective dose that produced the reduction of 50% of growth. The results showed that the genera Fusarium sp., Aspergillus sp., Mucor sp., Penicillium sp. and Sterilia sp. were the prevalent fungi isolated from soils both exposed and no-exposed to pesticides The highest value (>100 mM) of effective concentration of herbicide that produce the 50% of growth inhibition (EC50), was found for Trichoderma isolates. Sterilia spp. had EC50 values of 100 mM, while Aspergillus spp. and Mucor spp. had EC50 values between 50 and 100 mM. The growth rate evaluation varied according to the isolates and GP concentrations. The data showed that all Aspergillus spp., Trichoderma spp., Mucor spp and three Sterilia spp. had the best growth performance in media supplied with GP after a variable acclimation period. This study provide valuable data for further studies that allow knowing the metabolic capacity of these fungal species that can be potential candidates for removal of GP in contaminated environments.