Negative modulation of SA-signaling components by the capsid protein of Tobacco Mosaic Virus is required for viral long-distance movement

ANDREA VENTURUTZZI; MARIA CECILIA RODRIGUEZ; CONTI GABRIELA; LEONE, MELISA; CARO, MARÍA DEL PILAR; MONTECCHIA, JUAN; ZAVALLO, DIEGO; ASURMENDI SEBASTIAN

PLANT JOURNAL

WILEY-BLACKWELL PUBLISHING, INC

Lugar: Londres; Año: 2021

0960-7412

An important aspect of plant-virus interaction is the way viruses dynamically move over long distances and how plant immunity modulates viral systemic movement. Salicylic acid (SA), a well-characterized hormone responsible for immune responses against virus, is activated through different transcription factors including TGA and WRKY. In Tobamoviruses, evidences suggest that capsid protein (CP) is required for long-distance movement, although its precise role has not been fully characterized yet. Previously, we showed that the CP of TMV-Cg negatively modulates the SA-mediated defense. In this study, we analyzed the impact of SA-defense mechanism on the long-distance transport of a truncated version of TMV (TMV ∆CP virus) that cannot move to systemic tissues. The study showed that the negative modulation of NPR1 and TGA10 factors allows the long-distance transport of TMV ∆CP virus. Moreover, we observed that the stabilization of DELLA proteins promotes TMV ∆CP systemic movement. We also characterized a group of genes, part of a network modulated by CP, involved in TMV ∆CP long-distance transport. Altogether, our results indicate that CP mediated downregulation of SA signaling pathway is required for the virus systemic movement and this role of CP may be linked to its ability to stabilize DELLA proteins. Significance statement: TMV CP interfere with the immune system of plants, and this negative modulation allows the virus to move systemically. Specific signaling players (DELLA, NPR1 and TGA10) are involved in the regulation of TMV systemic movement, indicating that this process is highly regulated.