INVESTIGADORES
MAGNOLI Carina Elizabeth
artículos
Título:
Occurrence of ochratoxin A- producing fungi in commercial corn kernels in Argentina.
Autor/es:
MAGNOLI C.,; HALLAK C.,; CHIACCHIERA S.,; DALCERO A.
Revista:
MYCOPATHOLOGIA
Editorial:
SPRINGER
Referencias:
Lugar: 2006, 161:53-58.; Año: 2006 vol. 161 p. 53 - 58
ISSN:
0301-486X
Resumen:
Potentially ochratoxigenic Aspergillus and Penicillium species were identified and the natural occurrence of
ochratoxin A (OTA) in corn kernels was evaluated. Likewise, the capacity to produce OTA by AspergillusAspergillus and Penicillium species were identified and the natural occurrence of
ochratoxin A (OTA) in corn kernels was evaluated. Likewise, the capacity to produce OTA by AspergillusAspergillus
section Nigri and Circumdati was investigated. A total of 50 corn samples for human consumption was
collected in the south of Co´ rdoba Province. The surface-disinfected method for mycobiota determination
was used. The OTA detection was performed by HPLC. OTA production was tested in strains belonging to
section Nigri and Circumdati. Statistical analysis demonstrated that the specie A. flavus was isolated in
higher frequency (p<0.01) from corn kernels in DRBC and DG18 media. The percentage of corn kernels
contaminated by A. niger var. niger was similar in DRBC and DG18 media. The frequency of grains
contaminated by A. flavus and A. niger var. awamori was higher than A. niger var. niger and A. japonicusNigri and Circumdati was investigated. A total of 50 corn samples for human consumption was
collected in the south of Co´ rdoba Province. The surface-disinfected method for mycobiota determination
was used. The OTA detection was performed by HPLC. OTA production was tested in strains belonging to
section Nigri and Circumdati. Statistical analysis demonstrated that the specie A. flavus was isolated in
higher frequency (p<0.01) from corn kernels in DRBC and DG18 media. The percentage of corn kernels
contaminated by A. niger var. niger was similar in DRBC and DG18 media. The frequency of grains
contaminated by A. flavus and A. niger var. awamori was higher than A. niger var. niger and A. japonicusNigri and Circumdati. Statistical analysis demonstrated that the specie A. flavus was isolated in
higher frequency (p<0.01) from corn kernels in DRBC and DG18 media. The percentage of corn kernels
contaminated by A. niger var. niger was similar in DRBC and DG18 media. The frequency of grains
contaminated by A. flavus and A. niger var. awamori was higher than A. niger var. niger and A. japonicusp<0.01) from corn kernels in DRBC and DG18 media. The percentage of corn kernels
contaminated by A. niger var. niger was similar in DRBC and DG18 media. The frequency of grains
contaminated by A. flavus and A. niger var. awamori was higher than A. niger var. niger and A. japonicusA. niger var. niger was similar in DRBC and DG18 media. The frequency of grains
contaminated by A. flavus and A. niger var. awamori was higher than A. niger var. niger and A. japonicusA. flavus and A. niger var. awamori was higher than A. niger var. niger and A. japonicus
var. japonicus (p<0.01) in DG18 media. The other potentially ochratoxigenic species, A. ochraceus, was
isolated between 5% and 10% of the corn kernels in DG18 and DRBC media, respectively. The OTA
producing species P. verrucosum was not isolated. All samples of corn were OTA negative (<1 ng g)1).
Thirty strains (25%) of the black Aspergillus were OTA producers. From four strains of A. ochraceusjaponicus (p<0.01) in DG18 media. The other potentially ochratoxigenic species, A. ochraceus, was
isolated between 5% and 10% of the corn kernels in DG18 and DRBC media, respectively. The OTA
producing species P. verrucosum was not isolated. All samples of corn were OTA negative (<1 ng g)1).
Thirty strains (25%) of the black Aspergillus were OTA producers. From four strains of A. ochraceusP. verrucosum was not isolated. All samples of corn were OTA negative (<1 ng g)1).
Thirty strains (25%) of the black Aspergillus were OTA producers. From four strains of A. ochraceusAspergillus were OTA producers. From four strains of A. ochraceus
isolated, only one produced OTA. Due to the storage variable conditions could not be adequate in this
substrate, the presence of ochratoxigenic strains of section Nigri and OTA needs to be evaluated for a
longer time to establish the toxicological risk for human beings. The contamination of stored corn kernels
with A. flavus and Aspergillus section Nigri was significant.Nigri and OTA needs to be evaluated for a
longer time to establish the toxicological risk for human beings. The contamination of stored corn kernels
with A. flavus and Aspergillus section Nigri was significant.A. flavus and Aspergillus section Nigri was significant.