Genetic Diversity of Rice stripe necrosis virus and New Insights into Evolution of the Genus Benyvirus

BAGAYOKO, ISSIAKA; CELLI, MARCOS GIOVANNI; ROMAY, GUSTAVO; POULICARD, NILS; PINEL-GALZI, AGNÈS; JULIAN, CHARLOTTE; FILLOUX, DENIS; ROUMAGNAC, PHILIPPE; SÉRÉMÉ, DRISSA; BRAGARD, CLAUDE; HÉBRARD, EUGÉNIE

Viruses

MDPI

Año: 2021 vol. 13 p. 1 - 12

The rice stripe necrosis virus (RSNV) has been reported to infect rice in several countriesin Africa and South America, but limited genomic data are currently publicly available. Here,eleven RSNV genomes were entirely sequenced, including the first corpus of RSNV genomes ofAfrican isolates. The genetic variability was differently distributed along the two genomic segments.The segment RNA1, within which clusters of polymorphisms were identified, showed a highernucleotidic variability than did the beet necrotic yellow vein virus (BNYVV) RNA1 segment. Thediversity patterns of both viruses were similar in the RNA2 segment, except for an in-frame insertionof 243 nucleotides located in the RSNV tgbp1 gene. Recombination events were detected into RNA1and RNA2 segments, in particular in the two most divergent RSNV isolates from Colombia andSierra Leone. In contrast to BNYVV, the RSNV molecular diversity had a geographical structure withtwo main RSNV lineages distributed in America and in Africa. Our data on the genetic diversity ofRSNV revealed unexpected differences with BNYVV suggesting a complex evolutionary history ofthe genus Benyvirus.