Co-Occurrence of NDM-5 and RmtB in a Clinical Isolate of Escherichia coli Belonging to CC354 in Latin America

COSTA, AGUSTINA; FIGUEROA-ESPINOSA, ROQUE; GAUDENZI, FLORENCIA; LINCOPAN, NILTON; FUGA, BRUNA; GHIGLIONE, BARBARA; GUTKIND, GABRIEL; DI CONZA, JOSÉ

Frontiers in Cellular and Infection Microbiology

Frontiers Media SA

Año: 2021 vol. 11

New Delhi metallo-b-lactamase (NDM)-producing isolates are usually resistant to most blactamsand other antibiotics as a result of the coexistence of several resistance markers,and they cause a variety of infections associated to high mortality rates. Although NDM-1is the most prevalent one, other variants are increasing their frequency worldwide. In thisstudy we describe the first clinical isolate of NDM-5- and RmtB-producing Escherichia coliin Latin America. E. coli (Ec265) was recovered from a urine sample of a female outpatient.Phenotypical and genotypical characterization of resistance markers and conjugationassays were performed. Genetic analysis of Ec265 was achieved by whole genomesequencing. Ec265 belonging to ST9693 (CC354), displayed resistance to most blactams(including carbapenems), aminoglycosides (gentamicin and amikacin), andquinolones. Several resistance genes were found, including blaNDM-5 and rmtB, locatedon a conjugative plasmid. blaNDM-5 genetic context is similar to others found around theworld. Co-transfer of multiple antimicrobial resistance genes represents a particularchallenge for treatment in clinical settings, whereas the spread of pathogens resistantto last resort antibiotics should raise an alarm in the healthcare system worldwide.