In vitro inhibitory activity of IgY antibodies against Salmonella ser. Newport isolated from horses

BUSTOS C.; LEIVA C.; GAMBAROTTA M.; GUIDA N.; CHACANA P.

JOURNAL OF EQUINE VETERINARY SCIENCE

ELSEVIER SCIENCE INC

Lugar: Amsterdam; Año: 2021

0737-0806

Equine salmonellosis is caused mainly by Salmonella Typhimurium and Newport,which cause enterocolitis and diarrhea. Treatment usually includes the administrationof antibiotics. However, since multidrug-resistant Salmonella is commonly detected,alternative options to control the pathogen are needed. One of these options is the useof specific egg yolk antibodies (IgY) for passive immunotherapy. Thus, the aim of ourwork was to produce IgY antibodies against an equine S. ser. Newport strain andassess their in vitro inhibitory activity. To this end, laying hens were immunized with aninactivated S . ser. Newport strain by using either Freund´s or Montanide? adjuvantand egg yolk extracts were obtained. The levels of specific IgY antibodies againstSalmonella in sera and egg extracts were determined by dot-blot andmicroagglutination. Besides, the IgY extracts were characterized by total proteinanalysis, SDS-PAGE, Western Blot, and inhibition of bacterial motility. IgY extractsshowed high purity (87.7 to 91.8 %), high microagglutination titers, and the ability toinhibit the motility of the bacterium. The results using Montanide? were similar tothose using the traditional Freund´s adjuvant. Thus, Montanide? may also be a goodadjuvant to produce IgY. IgY-technology represents a potential tool for the control ofsalmonellosis in horses