Ischemic Preconditioning and Tacrolimus Pre-treatment As Strategies To Attenuate Intestinal Ischemia-Reperfusion Injury In Mice

PABLO STRINGA; ROMANIN, DAVID (1);; NATALIA LAUSADA; MACHUCA MARIANA; RAIMONDI CARLOS; CABANNE, ANA; MARTIN RUMBO; GONDOLESI GABRIEL

TRANSPLANTATION PROCEEDINGS

ELSEVIER SCIENCE INC

Lugar: Amsterdam; Año: 2013

0041-1345

The intestine is highly sensitive to ischemia-reperfusion injury (IRI), a phenomenon occurring in different intestinal diseases. Several strategies to mitigate IRI are in experimental stages; unfortunately, no consensus has been reached about the most appropriate one. We report a protocol to study ischemic preconditioning (IPC) evaluation in mice and to combine IPC and tacrolimus (TAC) pre-treatment in a warm ischemia model. Mice were divided in treated (IPC, TAC and IPC+TAC) and untreated groups before intestinal ischemia. IPC, TAC and IPC+TAC groups were able to decrease post-reperfusion nitrites levels (p