Volatile organic compounds profile synthesized and released by endophytes of tomato (Solanum lycopersici L.) and their antagonistic role

LÓPEZ, SILVINA M. Y.; GRACIELA N. PASTORINO; BALATTI, PEDRO A.

ARCHIVES OF MICROBIOLOGY

SPRINGER

Año: 2021

0302-8933

Aims: The endophytic microbiome uses mechanisms such as the secretion of diffusibleantibiotic molecules, synthesis and release of volatile organic compounds, and/ortoxins to protect plants. The aim of this research was to study the volatile organiccompounds (VOCs) profile as well as the diffusible secondary metabolites producedand released by endophytic bacteria isolated from tomato plants that in in-vitro assaysprevented growth of pathogenic fungi. Methods: Bacteria belonging to seven genera(Acinetobacter, Arthrobacter, Bacillus, Microbacterium, Pantoea, Pseudomonas, andStenotrophomonas) were isolated from different tissues of tomato plants with andwithout symptoms of Gray leaf spot, a disease provoked by Stemphylium lycopersici. Invitro, antagonistic assays were performed and the effect of volatile and solublecompounds released by endophytic bacteria on the growth of pathogenic fungi wasdetermined. The VOCs synthesized by the endophytes were extracted, identified andquantified. Results: These isolates representatives of seven bacterial genera inhibitedthe growth of three fungal pathogens of tomato S. lycopersici, Alternaria alternata andCorynespora cassiicola, which was related to the synthesis of soluble compounds aswell as VOCs. Conclusions: Endophytes synthesize and release different VOCs,probably due to the different type of interaction that each bacterium establishes withthe fungus, presenting a range of fungal growth inhibition.