INSIBIO   05451
INSTITUTO SUPERIOR DE INVESTIGACIONES BIOLOGICAS
Unidad Ejecutora - UE
artículos
Título:
Production, purification and characterization of serraticin A, a novel cold-active antimicrobial produced by Serratia proteamaculans 136
Autor/es:
L.A. SANCHEZ; M. HEDSTROM,; M.A. DELGADO; O.D. DELGADO
Revista:
JOURNAL OF APPLIED MICROBIOLOGY
Editorial:
WILEY-BLACKWELL PUBLISHING, INC
Referencias:
Lugar: Inglaterra; Año: 2010 vol. 109 p. 936 - 945
ISSN:
1364-5072
Resumen:
Abstract
Aim: This study focuses on the production, purification and characterization of
serraticin A, a novel cold-active antimicrobial produced by Serratia proteamac-
ulans 136.
Methods and Results: A Ser. proteamaculans strain producing a novel cold-
active antimicrobial was isolated from Isla de los Estados, Argentina. Antimi-
crobial production was optimized in a BIOFLO 101 bioreactor under batch
culture mode, with temperature, pH and dissolved oxygen controlled condi-
tions. A purification protocol was developed including activated charcoal
adsorption, solid-phase C18 extraction (SPE) and semi-preparative HPLC. The
molecular weight was determined by LC ⁄QTOF⁄MS⁄MS mass analysis.
Conclusions: Serratia proteamaculans 136 produces a cold-active low molecular
bacteriocin-like compound named serraticin A. In this work, it has been labo-
ratory-scale produced, purified and partially characterized. Cross-immunity test
revealed that serraticin A is very different from other well-known microcins
assayed, with a wide inhibitory spectrum, showing an interesting biotechnology
potential to be applied as a control agent against pathogenic bacteria.
Significance and Impact of the Study: The present study is the first report of a
cold-active compound with antimicrobial activity from Ser. proteamaculans.
The work also highlights that cold environments could be a suitable source of
micro-organisms with ability to produce cold-active biomolecules of biotechno-
logical interest.