Preparation of submicrometer monodisperse magnetic silica particles using a novel water in oil microemulsion: properties and application for enzyme immobilization

M.V. TUTTOLOMONDO; M.E. VILLANUEVA; ALVAREZ, GISELA SOLANGE; DESIMONE, M.; DIAZ, L

BIOTECHNOLOGY LETTERS

SPRINGER

Lugar: Berlin; Año: 2013 vol. 35 p. 1571 - 1577

0141-5492

The synthesis of monodispersed magnetic silica nanoparticles (MSN) is described using a water-in-oil reverse microemulsion system that does not require the use of co-surfactants. Sodium silicate, Tween 20 as a neutral surfactant and 1-butanol as the organic phase were used. There are several advantages of the proposed method including a saturation magnetization value of 10 emu/g for the particles obtained, uniformity of size and that they are easily functionalized to bind urease covalently. Moreover, the intra-day, inter-day and long-term stability results confirm that the procedure was successful and the enzyme-linked MSNs were stable over repeated uses and storage retaining more than 75 % activity after 4 months