INVESTIGADORES
SANTA MARIA guillermo Esteban
artículos
Título:
The HAK1 gene of barley belongs to a large gene family and encodes a high-affinity potassium transporter
Autor/es:
SANTA MARIA G.E.; RUBIO F; DUBCOVSKY J; RODRÍGUEZ-NAVARRO A
Revista:
PLANT CELL
Editorial:
AMER SOC PLANT BIOLOGISTS
Referencias:
Lugar: Rockville; Año: 1997 vol. 9 p. 2281 - 2289
ISSN:
1040-4651
Resumen:
The high-affinity K+ uptake system of plants plays a crucial role in nutrition and has been the subject of extensive kinetic studies. However, major components of this system remain to be identified. We isolated a cDNA from barley roots, HvHAKí, whose translated sequence shows homology to the Escherichia coli Kup and Schwanniomyces occidentalis HAK1 K+ transporters. HvHAKí conferred high-affinity K+ uptake to a K+-uptake-deficient yeast mutant exhibiting the hallmark characteristics of the high-affinity K+ uptake described for barley roots. HvHAK1 also mediated low-affinity Na+ uptake. Another cDNA (HvHAK2) encoding a polypeptide 42% identical to HvHAKl was also isolated.Analysis of several genomes of Triticeae indicates that HvHAKí belongs to a multigene family. Translated sequences from bacterial DNAs and Arabidopsis, rice, and possibly human cDNAs show homology to the Kup-HAK1-HvHAK1 family of K+ transporters.
