The Trypanosoma cruzi infection with an isolate obtained from endemic produce alterations in the complexes of the cardiac mitochondrial respiratory chain

BÁEZ ALEJANDRA; LO PRESTI SILVINA; RIVAROLA WALTER; GUZMAN GUSTAVO; COMAY DIEGO; PAGLINI PATRICIA

CIRCULATION

LIPPINCOTT WILLIAMS & WILKINS

Lugar: Philadelphia; Año: 2008 p. 243 - 243

0009-7322

Introduction: Reactive oxygen species have direct effects on cellular structure and function; mitochondria are damaged with the entrance of Trypanosoma cruzi into cardiac cells as a consequence of the immflamatory processes that provoke oxidative stress. Objective: In the present work we studied the effect of the T. cruzi infection upon the mitochondrial function measured by the enzymatic activity of respiratory chain complexes (CI ?CIV) in myocardium from mice infected with an isolate obtained from Santiago del Estero (endemic area). Materials and Methods: Albino Swiss mice (n= 30) were used and divided into the following groups, G1= non-infected (n=10); G2= infected with 50 trypomastigotes of Trypanosoma cruzi, SGO Z12 isolate (n=20)Cardiac tissue samples were obtained from both groups 10 and 30 days post infection (d.p.i.), (acute phase) and 365 d.p.i. (chronic phase). Mitochondria were isolated by subcelullar fractioning determining CI, CII, CIII and CIV enzymatic activity by spectrophotometry.Results: The enzymatic activities (μm.min-1/mg prot) obtained were: CI in G1=0.04±0.02, in G2 an increment was determined 10 d.p.i. and a significantly decrease at 30 and 365 d.p.i. (p