Structural and functional alterations of cardiac and skeletal muscle mitochondria in patients with congestive heart failure.

GUZMÁN MENTESANA GUSTAVO; BÁEZ ALEJANDRA; DOMÍNGUEZ RICARDO; CÓRDOBA ROQUE; LO PRESTI SILVINA; RIVAROLA WALTER; PONS PATRICIA; FRETES RICARDO; COMAY DIEGO; PAGLINI PATRICIA

CIRCULATION

Lippincott Williams & Wilkins (American Heart Association)

Lugar: EEUU; Año: 2008 p. 127 - 127

0009-7322

The heart is highly dependent on mitochondria for the energy required for contractility and other metabolic activities. Mitochondria represent 30% of the total volume of cardiomyocytes and provide almost 90% of their energy. Defects in mitochondrial structure and function have been associated to dilated and hypertrophic cardiomyopathies. Here we studied the structure and function of cardiac and skeletal muscle mitochondria obtained from patients with congestive heart failure (CHF) type III ?IV, to establish a possible correlation.Cardiac and skeletal muscle biopsies (left ventricle and pectoral muscle) were obtained from 10 patients during surgery; then were divided into: control (n:4): young patients with interauricular communication and normal ejection fraction; and patients with CHF type III-IV (n:6). Part of the samples were fixed and analyzed using a Zeiss electronic microscope. For functional activity mitochondria were isolated by subcelullar fractioning, determining the activity of the respiratory chain analyzing complex CIII by spectrophotometry. Protein concentrations were determined by the Bradford method. Statistical analysis were done by ANOVA and χ2.CHF patients presented less and smaller cardiac mitochondria, with disrupted membrane and disorganized cristae when compared to the control group (p