Role of mitochondria and reactive oxygen species in the progression of heart failure?.

GUZMÁN MENTESANA GUSTAVO; BÁEZ ALEJANDRA; CÓRDOBA ROQUE; DOMÍNGUEZ RICARDO; LO PRESTI SILVINA; RIVAROLA WALTER; PONS PATRICIA; FRETES RICARDO; PAGLINI PATRICIA

Revista de la Facultad de Ciencias Médicas de la Universidad Nacional de Córdoba

Revista de la Facultad de Ciencias Médicas de la Universidad Nacional de Córdoba

Año: 2010 vol. 67 p. 150 - 158

0014-6722

Congestive heart failure(CHF) would be associated with mitochondrial abnormalities and increased of reactive species of oxygen (ROS).To clarify these issues we studied the structure, function of the mitochondrial enzyme nitro oxide synthase inducible (iNOS) and lipoperoxidation of membranes, one of their products through the peroxide nitrite ion (ONOO-), in the heart muscle of patients with heart failure congestive (ICC) grade III and IV (according to New York Heart Association). We included 25 patients who underwent cardiovascular surgery to biopsies of the heart muscle. They were stratified into a group with CHF(n=18) and control group (n=7). In biopsies analyzed the enzymatic activity of mitochondrial complex III spectrophotometrically, which was measured in mM.ubiquinona-1.mg prot, while the mitochondrial morphology was analyzed by the Zeiss electronmicroscope, the areas were quantified with program Axionvision 4.6. Lipoperoxidation of membranes was measured by the presence of ONOO- by immunohistochemistry against primary antibody against 3-nitrotyrosine was used labkitsystem biogenic step to bidinbiotinperoxidase (SBA) and coloring triamiobencidina (TAB), it is made with semicuantificacion intensity SCORE test. The statistical estudies was ANOVA.The heart muscle of patients with CHF showed that the mitochondrial area was reduced by 78% compared with the control (160.37µm2 ± 9.87)(936.81µm2 ± 78.48) p