The role of the Caderins in the Apoptosis of porcine Granulosa Cells (CG)

MEDINA J; LOMBARDO DM.

BIOCELL

INST HISTOL EMBRIOL-CONICET

Lugar: Mendoza. Argentina; Año: 2007 vol. 31 p. 338 - 354

0327-9545

THE ROLE OF THE CADERINS IN THE APOPTOSIS OFPORCINE GRANULOSA CELLS (CG)Medina J, Lombardo DM.Cátedra de Histología y Embriología, Facultad de Cs. Veterinarias.UBA. E-mail: jmedina@fvet.uba.arThe normal physiology and the dynamics of the reproductive tissuedepends in great measure of an appropriate contact cell to cell,which is mediated by proteins of the cadherins family, moleculesCa++ dependent such as the E and N Cadherin. In these circumstances,the atresia process for apoptosis of the CG, it could berelated with the loss of this cellular contacts. In this work, the objectivewas to establish the relationship among the loss of the contactsCa++ dependent with the integrity and the apoptosis of theCG. The study model was a cellular primary culture of porcine CGobtained by aspiration of antrals follicles, which were treated withEGTA, to obtain the disruptión of the homophilic unions. The treatmentswere made with EGTA 9 nM and 100 nM and without EGTA.Simultaneously, the role of the depletion of serum was determinedmaking the same study in cultures with and without 5% SFB, bothtreatment at different times of induction 6, 12 and 24 hours.In each case, the apoptosis index (AI) was determined by DAPIand TUNEL techniques. Also, was carried out a quantitative evaluationby analysis of images of the expression of Bax and Bcl-2 byinmuno cytochemical (ICQ). To different concentration of the inductor,the treatments with serum demonstrated a bigger AI by 9nM (1,27; 2,58 and 1,38 to 6, 12 and 24 hs respectively). The activationwas significant in the treatments of 12 hs with serum and inthose of 6 hs without serum (p<0,05). In the treatments with serum,activation was observed respect to the control. The AI in thetreatments without serum was not significant. To concentrations of100 nM the AI didn’t demonstrate significant differences of activation.The quantitative determination of the expression of Baxand Bcl 2 for ICQ validates the previous results.