INVESTIGADORES
LOMBARDO Daniel Marcelo
artículos
Título:
a-Naphthoflavone induces Apoptosis in Porcine Granulosa Cells (PGC).
Autor/es:
GUARAGNA, C.; LOMBARDO, DM; PECCI, A; BARAÑAO, JL
Revista:
BIOCELL
Editorial:
INST HISTOL EMBRIOL-CONICET
Referencias:
Lugar: Mendoza. Argentina; Año: 2006 vol. 30 p. 515 - 515
ISSN:
0327-9545
Resumen:
<!-- /* Font Definitions */ @font-face {font-family:"Cambria Math"; panose-1:2 4 5 3 5 4 6 3 2 4; mso-font-charset:0; mso-generic-font-family:roman; mso-font-pitch:variable; mso-font-signature:-1610611985 1107304683 0 0 159 0;} /* Style Definitions */ p.MsoNormal, li.MsoNormal, div.MsoNormal {mso-style-unhide:no; mso-style-qformat:yes; mso-style-parent:""; margin:0cm; margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:12.0pt; font-family:"Times New Roman","serif"; mso-fareast-font-family:"Times New Roman";} h1 {mso-style-unhide:no; mso-style-qformat:yes; mso-style-link:"Título 1 Car"; mso-style-next:Normal; margin:0cm; margin-bottom:.0001pt; mso-pagination:widow-orphan; page-break-after:avoid; mso-outline-level:1; font-size:12.0pt; font-family:"Times New Roman","serif"; mso-font-kerning:0pt; font-weight:normal; font-style:italic;} p.MsoBodyText2, li.MsoBodyText2, div.MsoBodyText2 {mso-style-unhide:no; mso-style-link:"Texto independiente 2 Car"; margin:0cm; margin-bottom:.0001pt; text-align:center; mso-pagination:widow-orphan; font-size:9.0pt; font-family:"Times New Roman","serif"; mso-fareast-font-family:"Times New Roman";} span.Ttulo1Car {mso-style-name:"Título 1 Car"; mso-style-unhide:no; mso-style-locked:yes; mso-style-link:"Título 1"; mso-ansi-font-size:12.0pt; mso-bidi-font-size:12.0pt; font-style:italic;} span.Textoindependiente2Car {mso-style-name:"Texto independiente 2 Car"; mso-style-unhide:no; mso-style-locked:yes; mso-style-link:"Texto independiente 2"; mso-ansi-font-size:9.0pt; mso-bidi-font-size:9.0pt;} .MsoChpDefault {mso-style-type:export-only; mso-default-props:yes; font-size:10.0pt; mso-ansi-font-size:10.0pt; mso-bidi-font-size:10.0pt;} @page WordSection1 {size:595.3pt 841.9pt; margin:70.9pt 184.3pt 440.25pt 6.0cm; mso-header-margin:0cm; mso-footer-margin:0cm; mso-paper-source:0;} div.WordSection1 {page:WordSection1;} --> a-NAPHTHOFLAVONE INDUCES APOPTOSIS IN PORCINE GRANULOSA CELLS (PGC)   Guaragna C.1, Lombardo, DM.1, Pecci, A.2,3 y Barañao, JL.3,4 1 Fac.Cs. Veterinarias UBA,  2 Dpto. Química Biológica, FCEyN-UBA, 3 IFIByNE-CONICET, 4 IByME-CONICET.   a- naphthoflavone (a- NF) is an antagonist at the aryl hydrocarbon receptor (AhR), the cellular receptor of polycyclic aromatic hydrocarbons and dioxins, that inhibits AhR activity via a classical competitive binding mechanism. The AhR is involved in the metabolism of  a variety of environmental chemicals. AhR is part of an emerging family of ligand-activated transcriptional regulators that control a variety of target genes included the Cyp1A1. However, a- NF has been reported as an  AhR agonist depending on their concentration and cellular context. Recent findings indicates that the AhR may have a central role in ovarian physiology and regulate follicular growth during pre- and postnatal life. In bovines, the AhR may be involved in the progressing of oocytes maturation and in promoting the resumption of meiosis. This effect is blocked by a- NF. The aim of this work was to evaluate wether a- NF modulates the programmmed cell death of porcine granulosa cells. Primary cultures were obtained by aspirating  follicular fluid from 3-6 mm follicles. Cells were treated with a- NF (1 a 10 uM). Apoptosis was evaluated by chromatin staining with DAPI that shows the morphological changes typical of apoptosis and a dosis dependent induction. Detection of translocation of phosphatidylserine by annexin V binding indicated that cells died by apoptosis. The caspase 3 activity was asigned by the clivage of a colorimetric substrate and confirm the results mentioned above.