Immunohistochemical Screening of Cadherin Molecules during Folliculogenesis in Bovine Ovary

LOMBARDO, DM.; GUARAGNA, C.; SOÑEZ, MC.; VON LAWZEWITSCH, I.

BIOCELL

INST HISTOL EMBRIOL-CONICET

Lugar: Mendoza. Argentina; Año: 2004 vol. 28 p. 245 - 245

0327-9545

<!-- /* Font Definitions */ @font-face {font-family:"Cambria Math"; panose-1:2 4 5 3 5 4 6 3 2 4; mso-font-charset:0; mso-generic-font-family:roman; mso-font-pitch:variable; mso-font-signature:-1610611985 1107304683 0 0 159 0;} /* Style Definitions */ p.MsoNormal, li.MsoNormal, div.MsoNormal {mso-style-unhide:no; mso-style-qformat:yes; mso-style-parent:""; margin:0cm; margin-bottom:.0001pt; mso-pagination:widow-orphan; mso-layout-grid-align:none; punctuation-wrap:simple; text-autospace:none; font-size:10.0pt; font-family:"Times New Roman","serif"; mso-fareast-font-family:"Times New Roman"; mso-no-proof:yes;} p.MsoBodyText, li.MsoBodyText, div.MsoBodyText {mso-style-unhide:no; mso-style-link:"Texto independiente Car"; margin:0cm; margin-bottom:.0001pt; text-align:justify; mso-pagination:widow-orphan; tab-stops:right 402.0pt 540.0pt; mso-layout-grid-align:none; punctuation-wrap:simple; text-autospace:none; font-size:10.0pt; font-family:"Arial","sans-serif"; mso-fareast-font-family:"Times New Roman"; mso-ansi-language:EN-US;} p.MsoBodyText2, li.MsoBodyText2, div.MsoBodyText2 {mso-style-unhide:no; mso-style-link:"Texto independiente 2 Car"; margin-top:0cm; margin-right:0cm; margin-bottom:6.0pt; margin-left:0cm; line-height:200%; mso-pagination:widow-orphan; mso-layout-grid-align:none; punctuation-wrap:simple; text-autospace:none; font-size:10.0pt; font-family:"Times New Roman","serif"; mso-fareast-font-family:"Times New Roman"; mso-no-proof:yes;} span.TextoindependienteCar {mso-style-name:"Texto independiente Car"; mso-style-unhide:no; mso-style-locked:yes; mso-style-link:"Texto independiente"; font-family:"Arial","sans-serif"; mso-ascii-font-family:Arial; mso-hansi-font-family:Arial; mso-bidi-font-family:Arial; mso-ansi-language:EN-US;} span.Textoindependiente2Car {mso-style-name:"Texto independiente 2 Car"; mso-style-unhide:no; mso-style-locked:yes; mso-style-link:"Texto independiente 2"; mso-no-proof:yes;} .MsoChpDefault {mso-style-type:export-only; mso-default-props:yes; font-size:10.0pt; mso-ansi-font-size:10.0pt; mso-bidi-font-size:10.0pt;} @page WordSection1 {size:612.0pt 792.0pt; margin:70.85pt 3.0cm 70.85pt 3.0cm; mso-header-margin:36.0pt; mso-footer-margin:36.0pt; mso-paper-source:0;} div.WordSection1 {page:WordSection1;} --> IMMUNOHISTOCHEMICAL SCREENING OF CADHERIN MOLECULES DURING FOLLICULOGENESIS IN BOVINE OVARY. Guaragna C, Lombardo DM, Soñez MC, von Lawzewitsch I.   Cadherin molecules integrate a super family which participate in cell adhesion processes. Epithelial, neural and placental are classic cadherin molecules.  Epithelial (E-CAM) and neural (N-CAM) molecules were the most frequently found in embryonic and adult cells. Studies of E-CAM expression  in porcine ovaries have shown remarkable decrease during the ovary ontogeny. Actually there are not any reports about their expression related folliculogenesis in porcine and bovine ovaries. The aim of this work was to study the localization of E-CAM in the different follicular stages and their possible role in the follicular atresia.  The E-CAM expression was screening by immunohistochemistry (LSAB) using monoclonal anti-E-CAM 1:1600 (Santa Cruz Biotechn. Inc.) on heifer  ovary sections. The ovaries (n=120) included different developing follicular stages. The molecular expression was also determined in primary cultures of granulose cells by immunohistochemistry and western blot. E-cadherin was localized in the granulose cells of the antral wall of the pre-ovulatory and antral follicles while primordial and pre-antral follicles were immuno-negative independently their atretic or healthy conditions. The thecal and interstitial cells showed E-CAM immunoreactivity  which could indicate the expression would not restricted to cells of epithelial origin.  Intense and homogeneous immunoreactivity was found in surface ovary epithelium  in contrast the observations reported by other authors. In granulose primary cultures some clusters showed positive immunoreactivity while the most population resulted immuno-negative. Further studies would be necessary to determine the functions of the differential expression of cadherin molecules on the different cellular populations of the bovine granulose.