INVESTIGADORES
ASURMENDI Sebastian
artículos
Título:
Mucosal and systemic immunization elicited by Newcastle disease virus (NDV) transgenic plants as antigens.
Autor/es:
BERINSTEIN, A.; VAZQUEZ-ROVERE, C.; SEBASTIAN ASURMENDI; GÓMEZ, E.; ZANETTI, F.; ZABAL, O.; TOZZINI, A.; GRAND, D. C.; TABOGA, O.; CALAMANTE, G.
Revista:
VACCINE
Referencias:
Año: 2005 vol. 23 p. 5583 - 5589
ISSN:
0264-410X
Resumen:
As a step towards developing a safe and effective edible vaccine against Newcastle disease virus (NDV), we have explored the use of
plants genetically engineered to express viral proteins.We report the construction of transgenic potato plants expressing the genes coding for
immunogenic proteins of NDV under the regulation of CaMV 35S promoter and its immunogenicity in mice. All mice receiving transgenic
plant extracts in icomplete Freund adjuvant produced specific anti-NDV antibodies. Animals fed with transgenic leaves showed a specific
response aganist NDV. Detection of IgA released from in vitro-cultured intestinal tissue fragments indicated the presence of IgA-secreting
cells in the gut.