Snakin-1 affects reactive oxygen species and ascorbic acid levels and hormone balance in potato

VANESA NAHIRÑAK,; MAXIMO RIVAROLA; ALMASIA, NATALIA INÉS; BARRIOS BARON MP.;; ESTEBAN HOPP; DENIS VILE; NORMA PANIEGO; CECILIA VAZQUEZ ROVERE

PLOS ONE

PUBLIC LIBRARY SCIENCE

Lugar: San Francisco; Año: 2019

1932-6203

Snakin-1 is a member of the Solanum tuberosum Snakin/GASA family. We previously demonstrated that Snakin-1 is involved in plant defense to pathogens as well as in plant growthand development, but its mechanism of action has not been completely elucidated yet.Here, we showed that leaves of Snakin-1 silenced potato transgenic plants exhibitedincreased levels of reactive oxygen species and significantly reduced content of ascorbicacid. Furthermore, Snakin-1 silencing enhanced salicylic acid content in accordance with anincreased expression of SA-inducible PRs genes. Interestingly, gibberellic acid levels werealso enhanced and transcriptome analysis revealed that a large number of genes related tosterol biosynthesis were downregulated in these silenced lines. Moreover, we demonstratedthat Snakin-1 directly interacts with StDIM/DWF1, an enzyme involved in plant sterols biosynthesis. Additionally, the analysis of the expression pattern of PStSN1::GUS in potatoshowed that Snakin-1 is present mainly in young tissues associated with active growth andcell division zones. Our comprehensive analysis of Snakin-1 silenced lines demonstratedfor the first time in potato that Snakin-1 plays a role in redox balance and participates in acomplex crosstalk among different hormones.