Interferon-γ and IL-10 Release Assay for Patients with Ocular Toxoplasmosis

RUDZINSKI, MARCELO; PARDINI, LAIS; BERNSTEIN, MARIANA; MORÉ, GASTÓN; KHOURY, MARINA; DUARTE SZYSZKO, SILVANA CAROLINA; ARGÜELLES, CARINA; REINA, SILVIA; OUBIÑA, JOSÉ RAÚL

AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE

AMER SOC TROP MED & HYGIENE

Año: 2020

0002-9637

Peripheral blood mononuclear cells (PBMCs) from patients with ocular toxoplasmosis were challenged with Toxoplasma gondii lysate antigen (TLA) in a cytokine release assay (CRA), run during the inactive period of the disease. Increased interferon gamma (IFN-γ) levels were detected after PBMC stimulation with either ME49 reference strain (P = 0.0015) or local TgCkAr-11-9 isolate (P = 0.0012), as compared with those recorded under basal conditions. TLA from TgCkAr11-9 isolate induced a higher release of IFN-γ than ME49 strain CRA from all tested patients (P = 0.02). The median value of IFN-γ release on TgCkAr-11-9 stimulation (26.03 pg/mL) allowed the classification of patients into high? or low?/non?IFN-γ releasers. Clinical correlations were established with both groups. The results obtained in this study suggest the need to include local strains when performing CRA with T. gondii TLA.