Unraveling the genetic complexity of a cultivated breeding population of ?yerba mate? (Ilex paraguariensis St. Hil.)

ROSETTI, M.E.N.; PAIVA, D.I.; GAUCHAT, M.E.; CASCALES, J.; GOTTLIEB, A.M.

ANAIS DA ACADEMIA BRASILEIRA DE CIENCIAS.

ACAD BRASILEIRA DE CIENCIAS

Lugar: Rio de Janeiro; Año: 2020 vol. 92

0001-3765

We report the first comprehensive multilocus molecular characterization of cultivated Ilex paraguariensis plants belonging to a breeding program. Using nuclear and homologous chloroplastidic molecular markers, we have genotyped 158 plants from four plantation sites. Analyses of the nuclear data (187 variable dominant loci) allowed detecting high diversity (0.569), the occurrence of four distinct genetic clusters, and a low but significant differentiation among sites. Additionally, 20 chloroplastidic alleles were identified applying five microsatellite polymorphic markers, and a high chloroplastidic diversity was recognized (0.505); two haplogroups were distinguished amongst the 63 haplotypes detected. Our results from both nuclear and plastidic markers indicate that most genetic variation reside within plantations sites (≥ 95%), and that these plantations were established on highly variable materials (either as seeds or plantlets) derived from, at least, 63 maternal lineages. Moreover, our study suggests that the genetic structure within each plantation site was, not only, most likely shaped by the occurrence of past admixture favored by farmers´ practices during the establishment of each plantation, but also that some constraint in gene flow and/or a low level of shared polymorphism among plantations had occurred.