Gene gain and loss and recombination shape evolution of Listeria bacteriophages of the genus Pecentumvirus

DOLORES, BLANCO FERNANDEZ MARÍA; KLUMPP, JOCHEN; BARRIOS, MELINA E.; MBAYED, VIVIANA A.

GENOMICS

ACADEMIC PRESS INC ELSEVIER SCIENCE

Lugar: Amsterdam; Año: 2020 vol. 113 p. 411 - 419

0888-7543

Lis­te­ria mono­cy­to­genes is an im­por­tant food-borne pathogen and its bac­te­rio­phages are promis­ing tools for its con­trol in food and sur­faces. Lis­te­ria bac­te­rio­phages be­long­ing to the genus Pe­cen­tumvirus of the fam­ily Herelle­viri­dae are strictly lytic, have a con­trac­tile tail and a large dou­ble stranded DNA genome (mean of 135.4 kb). We re­port the iso­la­tion and genome se­quences of two new Pe­cen­tumvirus bac­te­rio­phages: vB_Lino_VEfB7 and vB_Li­va_­VAfA18. Twenty-one bac­te­rio­phages of this genus have been de­scribed and their genomes were used for the study of Pe­cen­tumvirus evo­lu­tion. Analy­ses showed collinear genomes and gene gain and loss propen­sity and re­com­bi­na­tion events were dis­tinctly found in two re­gions. A large po­ten­tial re­com­bi­na­tion event (≈20 kB) was de­tected in P100 and vB_Li­va_­VAfA18. Phy­lo­ge­netic analy­ses of multi-gene align­ments showed that di­ver­si­fi­ca­tion events formed two groups of species dis­tantly re­lated.