Occurrence of bacteria with technological and probiotic potential in Argentinian human breast-milk

ODDI, S.; BINETTI, A.; BURNS, P.; CUATRÍN, A.; REINHEIMER, J.; SALMINEN, S.; VINDEROLA, G.

Beneficial Microbes

Wageningen Academic Publishers

Año: 2020 vol. 11 p. 685 - 702

1876-2883

Breast milk provides not only essential nutrients and bioactive components to the infant gut but also cultivable and non-cultivable microbes. Lactobacilli and bifidobacteria occur naturally in breast milk and they are known for being beneficial for the neonate development and well-being. The aim of this study was to isolate lactobacilli from breast milk samples collected in Argentina, focusing on those isolates with promising functional and technological potential for the development of new probiotics. Fourteen Lactobacillus and one Bifidobacterium isolats were obtained from 164 breast milk samples donated by 104 mothers. The isolates L. gasseri (70a and 70c) and L. plantarum (73a and 73b) deserve further studies as potential probiotics due to their capacity to induce the release of IL-10 from murine macrophages and their high hydrophobicity. All the strains, except for L. gallinarum 94d, showed satisfactory survival to freeze-drying and long-term (12 months) storage at 5 °C. Lactose and polydextrose were satisfactory protectants for the technological application of these strains. Low correlation between survival to accelerated and long-term storage tests was observed.