BECAS
GATTI Cintia Romina
artículos
Título:
SOX-11 regulates LINE-1 retrotransposon activity during neuronal differentiation
Autor/es:
ORQUEDA, ANDRÉS J; GATTI, CINTIA R; OGARA, MARÍA F; FALZONE, TOMÁS L
Revista:
FEBS LETTERS
Editorial:
ELSEVIER SCIENCE BV
Referencias:
Lugar: Amsterdam; Año: 2018
ISSN:
0014-5793
Resumen:
Activity of the human long interspersed nuclear elements-1 (LINE-1) retrotransposon occurs mainly in early embryonic development and duringhippocampal neurogenesis. SOX-11, a transcription factor relevant to neuronaldevelopment, has unknown functions in the control of LINE-1 retrotransposon activity during neuronal differentiation. To study the dependence of LINE-1 activity on SOX-11 during neuronal differentiation, we induced differentiation of human SH-SY5Y neuroblastoma cells and adult adipose mesenchymal stem cells (hASCs) to a neuronal fate and found increased LINE-1 activity. We also show that SOX-11 protein binding to the LINE-1 promoter is higher in differentiating neuroblastoma cells, while knock-down of SOX-11 inhibits the induction of LINE-1 transcription in differentiating conditions. These results suggest that activation of LINE-1retrotransposition during neuronal differentiation is mediated by SOX-11.
