Enzyme Reaction in the Pores of CaCO3 Particles upon Ultrasound Disruption of Attached Substrate-Filled Liposomes

ALEXEY M. YASHCHENOK, MIHAELA DELCEA, KRISTINA VIDENOVA, ELIZABETH A. JARES-ERIJMAN, THOMAS M. JOVIN, MANFRED KONRAD, HELMUTH MÖHWALD, ANDRE G. SKIRTACH

Angewandte Chemie

WILEY-V C H VERLAG GMBH

Año: 2010 vol. 49 p. 8116 - 8120

1433-7851

Breaking out: Liposomes that are adsorbed onto porous CaCO3 particles and that contain the peroxidase substrate Amplex Red (AR) are disrupted by ultrasonic treatment. The substrate is released and diffuses into the inner part of the multicompartment containers where the peroxidase enzyme is found, and the enzymatic reaction is triggered. This approach may be useful for the simultaneous delivery of multiple molecules into cells.