EFFECT OF NATURAL PRODUCTS OF VEGETABLE ORIGIN AND SYNTHETIC PESTICIDES ON

ANA P. MEREP; ZELARAYAN, LILIANA; ADERA, MARIA; AJMAT MT; MATAS, ME

BIOCELL

- BIOCELL

Lugar: Tucuman; Año: 2019 vol. 43 p. 95 - 95

Synthetic pesticides (SP) application causes imbalances in ecosystems, so research on the insecticidal properties of bioactive natural products (NP) of vegetal origin has become relevant. One of the undesirable side effects of the indiscriminate use of SP is their ability to act as endocrine disruptors altering the reproductive functionality of humans and/or animals. Previous studies of our group have proven interesting lethal and sublethal effects of plant extracts obtained from regional flora on pest insects of economic importance. In this work, we comparatively evaluated the endocrine disruption capacity of natural products (NP) of known toxicity and commonly used SP with an in vitro maturation bioassay on Rhinella arenarum oocytes. Ovarian follicles obtained from R. arenarum females were exposed to NP or SP for 2 hours and maturation was induced with progesterone (P4) 10-6M. Germinal vesicle breakdown (GVBD) was controlled at 20 hours and experimental data were analyzed with Friedman test. NP used were pyrrolizidine alkaloids isolated from a methanol extract of Senecio rudbekiaefolious (100-400 mg/L) and aqueous and ethanolic extracts from Senecio eriophyton, Nicotiana noctiflora and Clinopodium gilliesii (250-1,000 mg/L); the SP chlorantranilprole and dimethoate were diluted from the concentrations used for application on foliage. Pretreatment of follicles with the tested NP did not affect the P4-induced oocyte maturation percentages (75-100% GVBD). In contrast, the SP significantly inhibited the P4-induced GVBD in a dose-dependent manner. These results provide a scientific basis for the implementation of these NP as potential biopesticides in the control of important pests in the region.