INVESTIGADORES
DELGADO Osvaldo Daniel
artículos
Título:
Production, purification and characterization of serraticin A, a novel cold-active antimicrobial produced by Serratia proteamaculans 136.
Autor/es:
SANCHEZ LA; HEDSTROM M; DELGADO MA; DELGADO OD
Revista:
JOURNAL OF APPLIED MICROBIOLOGY
Editorial:
WILEY-BLACKWELL PUBLISHING, INC
Referencias:
Año: 2010 vol. 109 p. 936 - 945
ISSN:
1364-5072
Resumen:
Aim: This study focuses on the
production, purification and characterization of serraticin A, a novel
cold-active antimicrobial produced by Serratia proteamaculans 136.
Methods and Results: A Ser.
proteamaculans strain producing a novel cold-active antimicrobial was isolated
from Isla de los Estados, Argentina. Antimicrobial production
was optimized in a BIOFLO 101 bioreactor under batch culture mode, with
temperature, pH and dissolved oxygen controlled condi-tions. A purification
protocol was developed including activated charcoal adsorption, solid-phase C18
extraction (SPE) and semi-preparative HPLC. The molecular weight was determined
by LC ⁄QTOF/MS/MS mass analysis.
Conclusions: Serratia proteamaculans
136 produces a cold-active low molecular bacteriocin-like compound named
serraticin A. In this work, it has been laboratory- scale produced, purified
and partially characterized. Cross-immunity test revealed that serraticin A is
very different from other well-known microcins assayed, with a wide inhibitory
spectrum, showing an interesting biotechnology potential to be applied as a
control agent against pathogenic bacteria.
Significance and Impact of the Study: The present
study is the first report of a cold-active compound with antimicrobial activity
from Ser. proteamaculans. The work also highlights that cold environments could
be a suitable source of micro-organisms with ability to produce cold-active
biomolecules of biotechnological interest.