PERSONAL DE APOYO
MUÑOZ Marina Cecilia
artículos
Título:
Behaviour of the anti-oxidant defense system and heme oxygenase-1 protein expresión in fructosa-hypertensive rats.
Autor/es:
ARIEL POLIZIO; SOLEDAD GONSALES; MARINA C MUÑOZ; CLARA PEÑA; MARÍA L TOMARO
Revista:
CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY
Editorial:
Wiley-Blackwell
Referencias:
Año: 2006 vol. 33 p. 734 - 739
ISSN:
0305-1870
Resumen:
1. Addition of fructose to a rat diet for various periods of time
leads to hypertension, hyperinsulinaemia and dyslipidaemia and
provides a model for testing oxidative stress parameters in the
animals.
2. In the present study, oxidative stress generation, the soluble
and enzymatic defence system and heme oxygenase-1 (HO-1)
protein expression were investigated in the heart, liver and kidney
of rats fed fructose for a period of 1 or 8 months.
3. Compared with the control group, fructose-hypertensive
rats showed increased in lipid peroxidation only in the heart
after both 1 and 8 months of fructose treatment. Changes in the
behaviour of the soluble and enzymatic defence system and
HO-1 protein expression were different depending on the organ.
Increased or unaltered activities of anti-oxidant enzymes were
found in the liver and kidney, respectively. Induction of HO-1
prevented the generation of oxidative stress in the liver, where
the activity of anti-oxidant defence enzymes was not reduced.
Increased expression of HO-1 protein was not able to prevent
the generation of oxidative stress in the heart, where fructose
treatment diminished the activity of anti-oxidant enzymes.
4. The results of the present study demonstrate that upregulation
of HO-1 may prevent the generation of oxidative stress
only when the anti-oxidant defence system is still operative.
Key words: anti-oxidant enzymes, fructose hypertension,
heme oxygenase, oxidative stress, rats.