Characterization of the key antigenic components of pertussis vaccine based on outer membrane vesicles

ORMAZABAL MAXIMILIANO; BARTEL ERIKA; GAILLARD MARÍA EMILIA; BOTTERO DANIELA; ERREA AGUSTINA; ZURITA EUGENIA; MORENO GRISELDA; RUMBO MARTÍN; CASTUMA CELINA; HOZBOR DANIELA

VACCINE

ELSEVIER SCI LTD

Lugar: Amsterdam; Año: 2014

0264-410X

EN REVISIONPertussis has resurged during the last two decades in different countries. In particular in2010-2013 period large outbreaks were detected in US, Australia, UK and The Netherlandswith significant mortality in infants. The epidemiological situation of pertussis point out theneed to develop new vaccines and in this sense we previously developed a new vaccinebased on outer membrane vesicle (OMV) which have been shown to be safe and to induceprotection in mice. We have here further investigated the properties of OMV vaccines; inparticular we investigated the contribution of pertussis toxin (PTx) and pertactin (Prn) inOMVs-mediated protection against pertussis. A PTx-deficient OMVs and Prn-deficientOMVs were obtained from defective B. pertussis mutants. The absence of PTx or Prn didcompromise the protective capacity of the OMVs formulated as Tdap vaccine. In vivo,whereas the protective efficacy of the PTx-deficient OMVs in mice was comparable to Prndeficient OMVs, the protective capacity of both of them was significantly impaired when itwas compared with the wild type OMVs. Interestingly, using OMVs obtained from B.pertussis strain which does not express any of the virulence factors but express the avirulentphenotype; we observed that the protective ability of such OMVs was lower than that ofOMVs obtained from virulent B. pertussis phase. However, it was surprising that althoughthe protective capacity of avirulent OMVs was lower, they were still protective in the usedmice model. These results allow us to hypothesize that OMVs from avirulent phase sharesprotective components with all OMVs assayed. Using immune proteomic strategy weidentified some common components could play an important role in protection againstpertussis.