Preliminary studies of new strains of Trametes sp. from Argentina for laccase production ability

FONSECA MI; TEJERINA MR; SAWOSTJANIK-AFANASIUK SS; GIORGIO EM; BARCHUK ML; ZAPATA PD; VILLALBA LL

BRAZILIAN JOURNAL OF MICROBIOLOGY

SOC BRASILEIRA MICROBIOLOGIA

Año: 2016

1517-8382

Oxidative enzymes secreted by white rot fungi can beapplied in several technological processes within the paper industry, biofuelproduction and bioremediation. The discovery of native strains from thebiodiverse Misiones (Argentina) forest can provide useful enzymes forbiotechnological purposes. In this work, we evaluated the laccase and manganeseperoxidase secretion abilities of four newly discovered strains of Trametes sp.that are native to Misiones. In addition, the copper response and optimal pHand temperature for laccase activity in culture supernatants were determined.The selected strains produced variable amounts oflaccase and MnP; when Cu2+ was added, both enzymes weresignificantly increased. Zymograms showed that two isoenzymes were increased inall strains in the presence of Cu2+. Strain B showed the greatestresponse to Cu2+ addition, whereas strain A was more stable at theoptimal temperature and pH. Strain A showed interesting potential for futurebiotechnological approaches due to the superior thermo-stability of itssecreted enzymes.