Cellulolytic ability of a novel promising Irpex lacteus (Basidiomycota: Polyporales) strain from the subtropical rainforest of Misiones province, Argentina

GIORGIO EM; VILLALBA LL; ROBLEDO GL; ZAPATA PD; SAPARRAT MCN

REVISTA DE BIOLOGíA TROPICAL

REVISTA DE BIOLOGIA TROPICAL

Lugar: Turrialba; Año: 2018

0034-7744

Abstract: The cellulolytic activity of fungi growing in thesubtropical rainforest of Misiones (Argentina) represents a challenge in thetechnological development of the production of cellulosic bioethanol in theregion using native sources. These fungi are promising to obtain sustainable enzymecocktails using their enzymes. Cellulolytic ability of 22 white-rot fungiisolated from the subtropical rainforest of Misiones-Argentina in agar mediumwith two types of cellulosic substrates, carboxy-methylcellulose or crystallinecellulose, were comparatively analyzed, and the activity of two cellulolyticenzymes was evaluated in liquid medium. Although all isolates were able to growand degrade both substrates in agar medium, and to produce total cellulaseFilter paper (FPase) and endo-B-1,4-glucanase (EG) activities in broth, theisolate Irpex sp. LBM 034 showed thegreatest enzymatic levels (FPase, 65.45 U L-1; EG, 221.21 U L-1).Therefore, the ITS sequence of this fungus was sequenced and analyzed through aphylogenetic analysis. These results indicate that the isolate LBM 034,corresponding to Irpex lacteus, has apromising cellulolytic ability and enzymes such as EG useful in sustainablesaccharification of cellulosic materials in the region.