Bacterial viability after antimicrobial photodynamic therapy with curcumin on multiresistant Staphylococcus aureus

PINTO, JULIANA G; PEREIRA, ANDRÉ HC; FERREIRA-STRIXINO, JULIANA; FREITAS, MIRIAN AA; CASAS, ADRIANA

FUTURE MICROBIOLOGY

FUTURE MEDICINE LTD

Año: 2019 vol. 14 p. 739 - 748

1746-0913

Staphylococcus aureus are multiresistant pathogens that causes superficial and systemic infections. Antimicrobialphotodynamic therapy (APDT) is an alternative in the treatment of diseases caused by thesebacteria. Aim: In this study the APDT response on growth, viability, formation of reactive oxygen speciesand adhesion of methicillin sensitive strains of Staphylococcus aureus, methicillin resistant strains of S.aureus and ATCC of S. aureus were evaluated in vitro, after incubation with Curcumin for 20 min, and irradiatedwith LED.Materials & methods: Bacterial growth was assessed by the number of colony formingunits, viability and adhesion were evaluated by confocal microscopy and ROS quantification was performedby fluorimetry. Results: Was observed increase in the production of ROS in APDT groups, besidesa decrease in the 4 log growth and loss of the bacterial adhesion. Conclusion: APDT with Curcumin maybe an interesting therapeutic alternative, due to its in vitro response, in the control multiresistant clinicalS. aureus strains.