INVESTIGADORES
MORILLA Maria Jose
artículos
Título:
Structural features of ultradeformable archaeosomes for topical delivery of ovalbumin
Autor/es:
DOLORES C. CARRER, LETICIA H. HIGA, MARIA VICTORIA DEFAIN TESORIERO, MARIA JOSE MORILLA, DIANA I RONCAGLIA AND EDER LILIA ROMERO.
Revista:
COLLOIDS AND SURFACES B-BIOINTERFACES
Editorial:
ELSEVIER SCIENCE BV
Referencias:
Lugar: Amsterdam; Año: 2014 vol. 121 p. 281 - 289
ISSN:
0927-7765
Resumen:
The ultradeformable archaeosomes (UDA, made of total polar archaeolipids (TPA) extracted from the extreme halophile archaea Halorubrum tebenquichense:soybean phosphatidylcholine (SPC):sodium cholate (NaChol), 3:3:1 w:w), are promising topical adjuvants showing high deformability, an essential property for intact skin penetration up to the viable epidermis/dermis. To gain insights on UDA structure, the interactions between TPA, SPC and the edge activator NaChol, were assessed by electro-spray ionization mass spectroscopy (ESI-MS) and confocal fluorescence microscopy of giant unilamellar vesicles (GUV). The non covalent heterodimers NaChol-SPC, NaChol-phosphatidylglycerophosphatemethyl ether (PGPMe), NaChol-sulfated diglycosyl diphytanylglycerol diether (SDGD5) and SPC-PGPMe detected in the gas phase by ESI-MS after direct infusion of UDA, together with the homogeneous parti-tion of FASTDiO and DiIC18 in GUV suggested that in these proportions, lipids and NaChol were miscible.We propose therefore, a model where in UDA the SPC diluted sufficient enough in the rich PGPMe TPA,so as to the low lateral mobility of molecules (typical of rich in PGPMe bilayers) was no longer experi-enced. We also found that 50 m deep within in vitro human skin canyons, the fluorescence of Alexa fluor647-ovalbumin in UDL was 1.5 folds higher than in UDA, indicating a potential steric hindrance of the voluminous structure of PGPMe UDA bilayer, to the penetration of a particulate cargo such as the 7 nm diameter ovalbumin. According to these observations, a further reduction in PGPMe, a lipid playing no immune role, content could help to improve the performance of UDA as topical adjuvants.