INVESTIGADORES
MORILLA Maria Jose
artículos
Título:
Ultradeformable archaeosomes as new topical adjuvants
Autor/es:
HIGA LETICIA H., SCHILRREFF PRISCILA, PEREZ ANA PAULA, IRIRARTE MAIARA A., RONCAGLIA DIANA I., MORILLA MARIA JOSE, ROMERO EDER L
Revista:
NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE
Editorial:
ELSEVIER SCIENCE BV
Referencias:
Lugar: Amsterdam; Año: 2012 vol. 8 p. 1319 - 1328
ISSN:
1549-9634
Resumen:
Ultradeformable archaeosomes (UDA) are vesicles made of soybean phosphatidylcholine (SPC), sodium cholate (NaChol) and polar lipids from Halorubrum tebenquichense (3:1:3 wt/wt). Although ultradeformable liposomes (UDL, made of SPC and NaChol at 6:1 wt/wt) and UDA were neither captured nor caused cytotoxicity on keratinocytes, UDA was avidly captured by macrophages, their viability being reduced by 0.4-1.6 mg/mL phospholipids by 25 to 60%. Instead, UDL were poorly captured and caused no toxicity. Balb/C mice immunized by the topical route with four doses of ovalbumin (OVA)-loaded UDA, at 75 μg OVA/600 μg phospholipids (125 nm mean size and -42 mV zeta potential), induced IgG titers tenfold to 100-fold higher than those immunized with OVA-loaded UDL at the same dosage. Both matrices penetrate to the same skin depth (nearly 10 μm after 1 hour on excised human skin), being the higher topical adjuvancy and higher phagocytic uptake of UDA related to its glycolipid content